DEAE Sepharose FF
DEAE Sepharose FF is weak ion exchange chromatography media with excellent flow properties and high capacity for proteins of all pI values. The ion exchange group is a diethylaminoethyl group which remains charged and maintains consistently high capacity over the entire working range, pH3-9.
Gel characteristics
|
Type of exchanger |
Weak anion |
|
Total ionic capacity |
0.13-0.17m mol/ml gel |
|
Available capacity |
thyroglobulin(TG)MW669000 2mg/ml；HAS MW68000 170mg/ml；α-lactalbumin MW 14300 150mg/ml |
|
Bead structure |
6%highly cross-linked Sephrose |
|
Bead size range |
45-165um |
|
Mean particle size |
90um |
|
Max. flow rate |
300cm/h |
|
Max. operating pressure |
0.015Mpa(0.15bar，2psi) |
|
pH stability |
Long term:3-12 short term :2-14 |
|
Chemical stability |
All commonly used aqueous buffers,1.0M NaOH, 8M Urea, 8M guanidine hydrochloride, ethanol, propanol and so on. |
|
Physical stability |
Negligible volume variation due to changes in pH or ionic strength. |
|
autoclavable |
At 0.1 M NaCl, 121℃ for 30min. |
The available capacity is measured in a 0.5×5cm column in the linear flow rate of 300cm/h. The balance buffer is 0.05M Tris-HCl, pH 8.3 and the elution buffer contains 2M NaCl.
All ranges given are estimates based on our knowledge and experience. please note the following aspects:
(1) pH stability, long term refers to the pH interval where the gel is stable over a long period of time without adverse effects on its subsequent chromatographic performance.
(2) pH stability, short term refers to the pH interval for re-generation, cleaning-in-place and sanitization procedures.