DEAE Sepharose CL-6B


DEAE Sepharose CL-6B is weak ion exchange chromatography media with excellent flow properties and high capacity for proteins of all pI values. The ion exchange group is a diethylaminoethyl group which remains charged and maintains consistently high capacity over the entire working range, pH2-13.

Gel characteristics

 Type of exchanger

Weak anion

Total ionic capacity

0.13-0.17m mol/ml gel

Available capacity

thyroglobulin(TG)MW669000 2mg/ml;HAS MW68000 170mg/ml;α-lactalbumin MW 14300 150mg/ml

Bead structure

6%highly cross-linked Sepharose

Bead size range


Mean particle size


Max. flow rate


Max. operating pressure


pH stability

Long term:3-12 short term :2-14

Chemical stability

All commonly used aqueous buffers,1.0M NaOH, 8M Urea, 8M guanidine hydrochloride, ethanol, propanol and so on.

Physical stability

Negligible volume variation due to changes in pH or ionic strength.


At 0.1 M NaCl, 121℃ for 30min.

The available capacity is measured in a 0.5×5cm column in the linear flow rate of 300cm/h. The balance buffer is 0.05M Tris-HCl, pH 8.3 and the elution buffer contains 2M NaCl.

All ranges given are estimates based on our knowledge and experience. Please note the following aspects:

(1) pH stability, long term refers to the pH interval where the gel is stable over a long period of time without adverse effects on its subsequent chromatographic performance.

(2) pH stability, short term refers to the pH interval for re-generation, cleaning-in-place and sanitization procedures.