M-MLV Reverse transcripatase


Moloney Murine Leukemia Virus Reverse Transcriptase(M-MLV RT) is isolated from recombinant E.coli strain expressing the gene of M-MLV. The recombinant protein is obtained by column purification.

M-MLV (RNase H-) lacks the activity of RNase H for multiple point mutations. It has the same activity of DNA polymerase as wild type, but has obviously enhanced extension capacity. The enzyme is used to synthesize long first-strand cDNA and construct overall length of cDNA library at high proportion. The optimal reaction temperature is 42