M-MLV Reverse transcriptase


Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV) uses Single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E coli expressing a portion of the pol gene of M-MLV on a plasmid.



5 × RT Buffer

Unit Definition:

Unit activity is calculated assuming a specific enzyme activity of 350,000 units per mg protein. Protein is determined by a modification of the Lowry method, using BSA as a standard.

One unit of M-MLV incorporates 1 nmol dTTP into acid-precipitable material in 10 minutes at 37