Sepharose FF
The highly cross-linked agarose beads of Sepharose FF are secondary polymerized. So the mechanical strength has been greatly improved. It is characterized by fast flow, so it was named “Fast Flow” and fit for industrial-scale production. The non-specific binding is low and the protein recovery is very high. It can be used in various chaotropic agents and organic solvents because of the high physical and chemical stability. It can also be cleaned in placed with 1-2M NaOH.
Table: Performance
Name |
Fractionation range/Da |
pH stability |
Particle size/μm |
Max. operating pressure/Mpa |
Sepharose FF |
6×104—3×107 |
3—13 |
50—150 |
0.1 |