AMV Reverse Transcriptase
Description:
AMV Reverse Transcriptase (AMV RT) is isolated from avian myeloblastosis virus. Molecularweight of αβ holoenzyme is 157KD. The enzyme is used to synthesize cDNA and sequencingRNA and DNA. AMV RT catalyzes the polymerization of DNA using total RNA or polyA+ RNA. The enzyme possesses an intrinsic RNase H activity for the degradation of RNA withinRNA/DNA heterozygote. Activating agent for AMV RT is Mg2+ or Mn2+ and its optimal reaction temperature is 42