CM Sepharose CL -6B
CM Sepharose CL -6B is a weak cation exchanger with excellent flow properties and high capacity for proteins of all pI values. The ion exchange group is a carboxyl methyl group which remains charged and maintains consistently high capacity over the entire working range, pH6-12.
Table：Medium characteristics
Type of ion exchanger: Weak cation
|
Total ionic capacity |
0.10-0.14mM H+ |
|
Available capacity |
IgG(MW 16000) 9.5mg/ml(Bovine) |
|
Bovine COHb (MW69000) 75mg/ml |
|
|
Ribonuclease（MW 137000） 120mg/ml |
|
|
Bead structure |
6% cross-linked agarose |
|
Bead size range |
45-165um |
|
Mean particle size |
90um |
|
Max. linear flow rate |
150cm/h at25℃，HR16/10 column，5cmbed height |
|
pH working range |
6-12 |
|
Chemical stability |
Long term:3-12；Onsite cleaning:2-14 |
|
Physical stability |
All commonly used aqueous buffers: 1.0M acetic acid，1.0M Na0H, 8M urea，8M guanidine hydrochloride, ethanol, methanol et al. |
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autoclavable |
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